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KMID : 0379120140420030191
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Korean Journal of Mycology
2014 Volume.42 No. 3 p.191 ~ p.200
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Diversity, Saccharification Capacity, and Toxigenicity Analyses of Fungal Isolates in Nuruk
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Kim Min-Sik
Kim Sin-Il
Ha Byeong-Seok
Park Hye-Young
Baek Seong-Yeol
Yeo Soo-Hwan
Ro Hyeon-Su
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Abstract
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Nuruk samples collected from various regions in Korea were investigated in terms of fungal contents and diversity. In measurement of colony forming unit (CFU) in Nuruk suspensions on DRBC agar, Nuruk samples MS4, MS8, and MS10 were among the highest fungal density, with 1,278.9±21.6 (×10), 1,868.0±27.7 (×10), and 775.1±19.2 (×10) were among the samples showing the highest fungal density. CFU per 20 mg Nuruk, respectively. The majority of fungal components were yeasts, including , , , and , whereas and , the representative Nuruk fungi, were predominant only in the low fungal density Nuruks (MS2, MS5, and MS11). Saccharification capability of the fungal isolates was assessed by measurement of amylase activity in the culture broth. The highest amylase activity was found in and , followed by . and showed fair amylase activity but significantly lower than those of the three fungal species. was suggested to play an additional role in degradation of β-glucan in crop component of Nuruk since was the only fungus that showed β-glucanase activity among the fungal isolates. To confirm the safety of Nuruk, aflatoxigenicity of the isolated was estimated using the DNA markers , , and . All of the isolates turned out to be non-aflatoxigenic as evidenced by the deletion of gene markers, and , and the absence of aflatoxin in the culture supernatants shown by TLC analysis.
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KEYWORD
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Aflatoxin, Fungal diversity, Nuruk, Saccharification, Yeast
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